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Image Search Results
Journal: Kidney International Reports
Article Title: Proteomic Analysis of Circulating IgA1-Containing Immune Complexes in Patients with IgA Nephropathy
doi: 10.1016/j.ekir.2025.07.005
Figure Lengend Snippet: Serum proteomics workflow for the isolation and analysis of the molecular-form proteomes of IgA1. IgA1-containing immune complexes (IgA1-IC) from sera of 20 patients with IgA nephropathy (IgAN) and 20 healthy controls (HCs) were isolated by lectin-affinity chromatography followed by size-exclusion chromatography (SEC) to separate IgA1-IC from monomeric and polymeric IgA1 (I–III). IgA-specific protease and liquid chromatography mass spectrometry (LC-MS) sequence-grade trypsin digestion were followed by LC-MS (IV–V). After standard proteomic database searches, LC-MS results were curated by Scaffold perSPECtives to identify proteins of high abundance in IgA1-IC fractions of serum from patients with IgAN versus IgA1-IC from HCs with label-free quantification (LFQ). Additional comparisons were made to polymeric IgA1 (VI). Created with BioRender.com .
Article Snippet: Qualitative and quantitative comparisons of proteins identified in chromatographic fractions with different molecular forms of IgA1 were performed using
Techniques: Isolation, Affinity Chromatography, Size-exclusion Chromatography, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Sequencing, Quantitative Proteomics